Low-Frequency Mechanical Stimulation Modulates Osteogenic Differentiation of C2C12 Cells
Figure 3
(a) Experimental protocol A involved BMP-2 induction for 48 hours with mechanical stimulation only for the latter 24 hours. Controls were BMP-2-induced for 48 hours in the absence of any mechanical stimulation. (b) Mechanical stimulation in the presence of BMP-2 enhanced osteogenic gene expression versus unstimulated controls. Mean ± SEM, ; *represents ; #represents . (c) Morphology of C2C12 cells after 48 hours in experimental cultures (top) and controls (bottom). Scale bar represents 200 μm.