Table of Contents
ISRN Infectious Diseases
Volume 2013, Article ID 154921, 3 pages
Research Article

Prevalence of Metallo-β-Lactamases Producing Acinetobacter baumannii in a Moroccan Hospital

1Laboratory of Microbiology, Hospital of Specialties, Ibn Sina University Hospital, Rabat, Morocco
2Faculty of Medicine and Pharmacy, University Mohammed V Souissi, Rabat, Morocco
3Intensive Care Unit, Hospital of Specialties, Ibn Sina University Hospital, Rabat, Morocco

Received 12 September 2012; Accepted 8 October 2012

Academic Editors: A. Carvalho and A. Pop-Vicas

Copyright © 2013 Hakima Kabbaj et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To determine the prevalence of metallo beta-lactamases (MBL) among carbapenem resistant strains of Acinetobacter baumannii in our hospital. Methodology. During a period of 12 months (January–December 2010), 47 isolates of Acinetobacter baumannii were collected from different clinical specimens of in-patients. Antimicrobial susceptibility was determined and interpreted using the disk diffusion method according to the Antibiogram Committee of the French Society for Microbiology guidelines. Imipenem nonsusceptible isolates were further screened for production of MBL. Results. All Acinetobacter baumannii’ isolates were resistant to ticarcillin, ticarcilline/clavulanate, piperacillin, piperacillin/tazobactam, gentamicin, tobramycin, and ciprofloxacin, except an isolate that was sensitive to ceftazidime and cefepime. In addition to that, amikacin and trimethoprim/sulfamethoxazole were, respectively, sensitive by 59.5% and 53%. Among 57,4% (27/47) imipenem non-susceptible isolates of Acinetobacter baumannii, 74% (20/27) were found to be MBL producers. Conclusion. Although the rate of imipenem non-susceptible isolates of Acinetobacter baumanni seems to remain stable in 2005 (57%) and 2010 (57,5%), the prevalence of MBL producer strain is increasing (38% in 2005 versus 75% in 2010). The findings strongly suggest that there is a need to track the detection of MBL producers; moreover, a judicious use of carbapenems is necessary to prevent further spread of these organisms.