International Scholarly Research Notices

Research Article

Use of PCR-DGGE Based Molecular Methods to Analyse Microbial Community Diversity and Stability during the Thermophilic Stages of an ATAD Wastewater Sludge Treatment Process as an Aid to Performance Monitoring

Table 1

List of the oligonucleotide primers utilized in this study.


Primer		Sequence (5′-3′)²	Specificity	Techniques	Reference
Taxonomic lineage	Name of primer	Sequence (5′-3′)²	Specificity	Techniques	Reference

Bacteria	27F¹	AGAGTTTGATCCTGGCTCAG	V1, 16S rDNA	PCR	[63]
	1492R¹	GGTTACCTTGTTACGACTT	V9, 16S rDNA	PCR	[63]
	GC-338F	ACTCCTACGGGAGG CAGCAG	16S rDNA	PCR, DGGE	[19]
	518R	ATTACCGCGGCTGCTGG	16S rDNA	PCR, DGGE	[19]
	GC-948F	AACGCGGAAGAACCTTAC	V6, 16S rDNA	PCR, DGGE	[39]
	L1401R	CGGTGTGTACAAGAAGACCC	V8, 16S rDNA	PCR, DGGE	[63]
	GC-rpoB1698F3	AACATCGGTTTGATCAAC	rpoB	PCR, DGGE	[24]
	rpoB2041R	CGTTGCATGTTGGTACCCAT	rpoB	PCR, DGGE	[24]

Fungi	F3F	TCCTCTAAATGACCAAGTTTG	18S rRNA	PCR	[13]
Fungi	EF4R	GGAAGGG[G/A]TGTATTTATTAG	18S rRNA	PCR	[13]

Archaea	Arc 21F	TTCCGGTTGATCCYGCCGGA	16S rRNA	PCR	[64]
Archaea	Arc 958R	YCCGGCGTTGAMTCCAATT	16S rRNA	PCR	[64]

Eukarya	Euk1427F	TCTGTGATGCCCTTAGATGTTCTGGG	18S rRNA	PCR	[65]
Eukarya	Euk1616R	GCGGTGTGTACAAAGGGCAGGG	18S rRNA	PCR	[65]

F: forward primer; R: reverse primer. The numbering denotes positions of primers relative to the E. coli 16S rDNA gene.
²GC clamp added to the 5′ end of the primer 338, 5′CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGG G 3′.
Sequences represent the nucleotide sequences in the GC clamps of the respective primers.