Mouse Models and Techniques for the Isolation of the Diabetic Endothelium
Figure 5
Flow cytometry analysis of monocyte marker expression in the sorted endothelial population. Cells from the skeletal muscle of ScN/GFP mice fed chow or HFD for 8 weeks were stained for the monocyte marker CD11b. (a) Percentage of all mononuclear cells that are GFP+/CD11b−, GFP+/CD11b+, or GFP−/CD11b+ are shown in gates P5–P7, respectively. (b) Percentage of the sorting population that are GFP+/CD11b− or GFP+/CD11b+ are indicated in gates P2 and P3. The sorting gate is established based on GFP+ fluorescent signal above non-GFP sample. There are no GFP−/CD11b+ cells within the sorting gate as shown in gate P4. Only GFP+ cells with no fluorescent signal for CD11b are present in unstained samples from ScN/GFP mice (not shown).
