Improvement of Polyunsaturated Fatty Acid Production in Echium acanthocarpum Transformed Hairy Root Cultures by Application of Different Abiotic Stress Conditions
Figure 2
Total lipid (TL) content (mg/g DW) of E1.5 Echium acanthocarpum transformed hairy root cell line cultured in different culture media and temperatures. Values represent the mean of three independent replicates . Depending on the growth temperature, the sampling points varied as T1, 5 days for culture B1 and 15 days for cultures C1–C4; T2, 10 days for culture B1 and 25 days for cultures C1–C4; T3, 15 days for culture B1 and 35 days for cultures C1–C4; T4, 20 days for culture B1 and 45 days for cultures C1–C4; T5, 25 days for culture B1 and 65 days for cultures C1–C4; and T6, 35 days for culture B1 and 75 days for cultures C1–C4.
