Improvement of Polyunsaturated Fatty Acid Production in Echium acanthocarpum Transformed Hairy Root Cultures by Application of Different Abiotic Stress Conditions

Table 10

Two-way ANOVA (stress conditions and sampling time) of PC1 and Trans-PC2 of fatty acid profiles in Echium acanthocarpum hairy roots, growing in different culture media at 15°C (cultures C1–C4).

Two-way ANOVA	Stress conditions(osmotic pressure and carbon source)		Time (sampling point)		Interaction between factors
	𝐹-value	Sign.	𝐹-value	Sign.	𝐹-value	Sign.
PC1	13.912	0.000	24.725	0.000	8.315	0.000
Trans-PC2	9.534	0.000	2.882	0.024	1.610	0.106

PC: principal component; Trans-PC2: transformed principal component 2 (NL (PC2-min (PC2) + 1)); Sign.: significance (𝑃≤0.05).