Response of Maize Seedlings to Cadmium Application after Different Time Intervals
Table 1
Mean sum of square from ANOVA of data for growth and biochemical attributes of two maize (Zea mays L.) lines under different treatments of cadmium after different time intervals.
S.O.V.
df
Shoot dry weight
Root dry weight
Leaf area
Chlorophyll a
Lines ()
1
15.56***
3.97***
2074.52***
4.64***
Cadmium (Cd)
4
5.88***
2.97***
227.06***
5.68***
Time ()
2
38.97***
13.64***
2109.14***
54.73***
4
1.33***
0.20***
53.27***
0.12***
2
0.61***
0.31***
2.85*
1.71***
8
0.21***
0.16***
1.69*
0.90***
8
0.44***
0.16***
18.06***
0.31***
Error
60
0.021
0.024
0.713
0.009
LSD 0.05
—
0.237
0.256
1.379
0.153
S.O.V.
df
Chlorophyll b
Total Chlorophyll
Carotenoids
RMP
Lines ()
1
2.19***
13.26***
0.57***
366.91***
Cadmium (Cd)
4
10.97***
32.45***
0.77***
3665.94***
Time ()
2
14.40***
112.56***
4.94***
14370.68***
4
0.13***
0.25***
0.01***
22.34***
2
0.12***
2.40***
0.16***
181.05***
8
0.77***
2.78***
0.12***
239.16***
8
0.10***
0.57***
0.04***
55.36***
Error
60
0.011
0.020
0.00026
2.578
LSD 0.05
—
0.171
0.233
0.026
2.622
S.O.V.
df
MDA
H2O2
TAA
Free proline
Lines ()
1
554.23***
117.31***
17.90***
102.86***
Cadmium (Cd)
4
270.68***
310.40***
3.93***
86.99***
Time ()
2
651.96***
834.04***
6.84***
89.23***
4
42.74***
3.53***
0.77***
1.46***
2
79.58***
105.45***
4.81***
6.63***
8
3.69**
6.84***
0.07***
2.95***
8
6.78***
7.03***
0.07***
0.31***
Error
60
1.030
0.143
0.017
0.061
LSD 0.05
—
1.657
0.618
0.211
0.403
S.O.V.
df
Anthocyanin
Lines ()
1
101.98***
Cadmium (Cd)
4
351.97***
Time ()
2
193.48***
4
8.04***
2
9.26***
8
3.16***
8
8.35***
Error
60
0.254
LSD 0.05
—
0.824
*,**,***: significant at 0.05, 0.01, and 0.001 levels, respectively; ns: nonsignificant. RMP: relative membrane permeability; MDA: malendialdehyde; H2O2: hydrogen peroxide; TAA: total free amino acids.
