Table of Contents
ISRN Endocrinology
Volume 2013, Article ID 181950, 7 pages
http://dx.doi.org/10.1155/2013/181950
Research Article

Impact of Neonatal Manipulation of Androgen Receptor Function on Endocrine-Metabolic Programming in the Juvenile Female Rat

1Neuroendocrine Unit, IMBICE (CICPBA-CONICET La Plata), 1900 La Plata, Argentina
2CENEXA (UNLP-CONICET La Plata, PAHO/WHO Collaborating Centre for Diabetes), Medical School, La Plata National University, Calles 60 y 120, 1900 La Plata, Argentina

Received 23 May 2013; Accepted 28 July 2013

Academic Editors: C.-H. Anderwald, E. Hajduch, M. Hara, R. Rey, and J. A. Rillema

Copyright © 2013 Luisina Ongaro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The impact of neonatal androgen receptor (AR) stimulation/blockage, due to testosterone propionate (TP)/AR antagonist treatment, on individual anthropometry and neuroendocrine-metabolic function was evaluated in the juvenile female rat. Pups (age 5 days) were s.c. injected with TP (1.25 mg), flutamide (F; 1.75 mg), and TP + F or vehicle (control, CT) and studied on day 30 of age. Body weight (BW), parametrial adipose tissue (PMAT) mass, food intake, adipoinsular axis, and steroidogenic functions were examined. Opposite to TP-rats, F-treated rats developed hypophagia, grew slowly (BW and PMAT), and displayed heightened peripheral insulin sensitivity. These F effects were abrogated in TP + F animals. Accordingly, TP rats displayed hyperleptinemia, an effect fully prevented by F cotreatment. Finally, androgen-treated animals bore an irreversible ovarian dysfunction (reduced circulating levels of 17HOP4 and ovary 17HOP4 content and P450c17 mRNA abundance). These data indicate that early stimulation of AR enhanced energy store, blockage of AR activity resulted in some beneficial metabolic effects, and neonatally androgenized rats developed a severe ovarian dysfunction. Our study highlights the important role of AR in the early organizational programming of metabolic and neuroendocrine functions.