Table of Contents
ISRN Chemical Engineering
Volume 2013, Article ID 186159, 5 pages
Research Article

Performance Comparison of α- and β-Amylases on Chitosan Hydrolysis

Department of Chemical Engineering, Universitas Diponegoro, Jl. Prof. Soedarto-Tembalang, Semarang 50275, Indonesia

Received 22 August 2013; Accepted 3 October 2013

Academic Editors: G. Bayramoglu and J. Lobato

Copyright © 2013 Nur Rokhati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The low solubility in common solvent and high viscosity resulting from its high molecular weight (MW) with fiber-like structure prevents a more widespread use of chitosan. This paper presents a performance comparison of nonspecific, commercially available enzymes, α- and β-amylases, for the hydrolysis of chitosan to lower its MW. The results showed that both enzymes demonstrate the ability to be used as catalysts in chitosan hydrolysis with β-amylase having better performance than α-amylase. The chitosan hydrolysis was influenced by not only the enzyme and the chitosan characteristics but also the hydrolysis condition. The optimum pH solution was 4 for α-amylase and 5 for β-amylase. The hydrolysis temperature was found to be optimal at 90 and 50°C for α- and β-amylases, respectively.