The Role of Interleukin-1 Genotype in the Association between Coronary Heart Disease and Periodontitis in a Syrian Population

Table 1

Genotyping of the four polymorphic variants.

Gene/SNP	Primer sequences	Ta; (°C)	RE	DT; (°C)	DD; (h)	PCR product; (bp)	HWE; P value
IL-1α +4845; rs17561; G>T	FW: 5′-ATGGTTTTAGAAATCATCAAGCCTAGGGCA-3′; Rev: 5′ATGAAAGGAGGGGAGGATGACAGAAATGT-3′	56	SatI	37	16	153	0.3124
IL-1β  +3954; rs1143634; C>T	FW: 5′-CTCAGGTGTCCTCCAAGAAATCAAA-3′; Rev: 5′-GCTTTTTTGCTGTGAGTCCCG-3′	57	TaqI	65	2	182	0.0591
IL-1β  −511; rs16944; A>G	FW: 5′-TGGCATTGATCTGGTTCATC-3′; Rev: 5′-GTTTAGGAATCTTCCCACTT-3′	55	AvaI	37	16	304	0.547
IL-1 RN VNTR	FW: 5′-CTCAGCAACACTCCTAT-3′; Rev: 5′-TCCTGGTCTGCAGGTAA-3′	55				410 = 4 repeats; 240 = 2 repeats	0.562

SNP: single nucleotide polymorphisms; Ta: annealing temperature; HWE: the Hardy-Weinberg equilibrium; RE: restriction enzyme; DT: digestion temperature; DD: digestion duration; rs numbers are RefSNP SNP-identification codes as applied in the public nucleic acid polymorphism databases at the national center for biotechnology information [34].