Analysis of CYP2D6 Allele Frequencies and Identification of Novel SNPs and Sequence Variations in Sardinians

Table 3

List of primers used in CYP2D6 sequencing Analysis. Forward and reverse primers were used to sequence both strands of PCR products.

Nucleotides position	Forward primers		Reverse primers
	Name	5′-sequence-3′	Name	5′-sequence-3′
From −1780 to −1426	P–1780	GTCCTCCTGTCCTCAGTGGAT	–1426C>T	GTGTGCCACCACGTCTAGCTTTTT
From −1584 to −1287	–1584C>G	CTGGACAACTTGGAAGAACC	CYP2D6_02_R	AGACGGAGATTTCCTCTTGTT
From −1584 to −1000			–1000G>A	ACATCCTCCCGGGCTGCCTGAGGGT
From −1201 to −740	CYP2D6_01_F	GGTGCCTGTAGTCCCAGCTA	–740C>T	ACAGACTCACACTGACACTTAG
From −750 to −327	–750_–749delGA	TGTGACTGGTGTGTGTGAGAGA	CYP2D6_03_R	TGGGATAACCAGGGTTTCAG
From −320 to 137	2D6–F [14]	CCAGAAGGCTTTGCAGGCTTCA	137_138insT	GAAGTCCACATGCAGCA
From 19 to 481	19G>A	CTGGGGCTAGAAGCACTG	CYP2D6_04_R	CGTGATTTAAACGGCACTCA
From 479 to 883	CYP2D6_02_F	GCTGACACCAGAAGGAAAGG	883G>C	TCCCCGAAGCGGCGCCGCAA
From 843 to 1388	843T>G	ACTAGGACCTGTAGTCTGGGG	P2x2R [18]	ACCGGATTCCAGCTGGGAAATG
From 1294 to 1707	CYP2D6_03_F	ACCTGCACTAGGGAGGTGTG	1707delT	GCGGCCTCCTCGGTCACCC
From 1513 to 1979	1513C>T	AGCTGGACAGAGCCAGGGACTG	1979T>C	GGACAGCCCGACTCCTCCTTC
From 1863 to 2291	1863_1864insTTTCGCCCC	GACGCCCCTTTCGCCCC	2291G>A	CACTCGCCAAGTGCCAGCCTCCAC
From 2127 to 2616	CYP2D6_04_F	TTTGGGAAAGGACATTCCAG	2615_2617delAAG	GGCAGCCACTCTCACCT
From 2540 to 2988	2539_2542delAACT	ACAGCTGGATGAGCTGCT	2988G>A	CATGTGCCCCCGCCTGTACCCTT
From 2850 to 3288	2850C>T	AGCTTCAATGATGAGAACCTG	3288G>A	GATGTCATATGGGTCACAC
From 3030 to 3494	CYP2D6_07_F	ATGAACTTTGCTGGGACACC	CYP2D6_05_R	CCAGCCCTGCCTATACTCTG
From 3466 to 3853	CYP2D6_05_F	TAGTCCTCAATGCCACCACA	3853G>A	GAAGCGGAAGGGCTTCT
From 3790 to 4180	3790C>T	CCACTCTCACCCTGCATCT	4180G>C	AGCTCATAGGGGGATGGG
From 4115 to 4401	4115C>T	ACCTCCCTGCTGCAGCACTT	4401C>T	TAACTGACATCTGCTCAGCCTCAAC
From 4265 to 4728	CYP2D6_06_F	TCCCTAGCCAGAGGCTCTAA	2D6–R [14]	ACTGAGCCCTGGGAGGTAGGTA