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ISRN Endocrinology
Volume 2013 (2013), Article ID 205385, 6 pages
Review Article

Comparison of In Vitro and In Situ Methods for Studying Lipolysis

1Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran
2Paramedical School of Langeroud, Guilan University of Medical Sciences, Langeroud 4193844937, Iran

Received 20 May 2013; Accepted 21 July 2013

Academic Editors: C. Fürnsinn, T. Kalamatianos, and C. G. Scanes

Copyright © 2013 Ahmad Ghorbani and Mahmood Abedinzade. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Lipolysis is a highly regulated process and is controlled by nervous system, hormones, and paracrine/autocrine factors. Dysregulation of lipolysis is associated with some pathophysiological conditions including diabetes, metabolic syndrome, and obesity. Nowadays, special attention isthereforepaid to study lipolysis using different experimental models. This review summarizes the current experimental methods for studying lipolysis. Culture of preadipocyte cell lines, use of differentiated stroma-vascular cells, primary culture of adipocyte, organ culture of adipose tissue, and microdialysis technique are the most widely used techniques to study lipolysis. The advantages and limitations of using these methods are discussed.