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ISRN Analytical Chemistry
Volume 2013 (2013), Article ID 209518, 9 pages
Research Article

Spectrophotometric Determination of Some Non-Sedating Antihistamines Using Erythrosine B

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

Received 9 April 2013; Accepted 12 May 2013

Academic Editors: A. Garcia Asuero, E. T. Haupt, B. K. Jena, J. B. MacMillan, and A. Senthil Kumar

Copyright © 2013 Michael E. El-Kommos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple and sensitive spectrophotometric method has been developed for the determination of cetirizine (I), ebastine (II), fexofenadine (III), ketotifen (IV), and loratadine (V) based on ion-pair complex formation with erythrosine B. The pink color of the produced complex was measured at 550 nm without solvent extraction. Appropriate conditions were established by studying the color reaction between erythrosine B and the studied drugs to obtain the maximum sensitivity. Beer-Lambert's law is obeyed in the concentration ranges 1–7, 1–8, and 1–6  g/mL for (I, IV), (II, III), and (V), respectively. The method was validated according to ICH guidelines. The suggested method is applicable for the determination of the five investigated drugs in bulk and pharmaceutical dosage forms with excellent recoveries.