Table of Contents
ISRN Tropical Medicine
Volume 2013 (2013), Article ID 230273, 7 pages
http://dx.doi.org/10.1155/2013/230273
Research Article

Soluble ST2 Does Not Regulate TNF- and IL-6 Production in Dengue Virus-Infected Human Monocytes

Instituto de Virología, Universidad El Bosque, Carrera 7B Bis No. 132-11, Bloque D, Bogotá 110121, Colombia

Received 25 April 2013; Accepted 28 May 2013

Academic Editors: F. Fouque, A. Talvani, and C. Wrenger

Copyright © 2013 Marisol Pérez-Acosta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dengue virus (DENV) produces an acute infection that results in the overproduction of proinflammatory cytokines. Although increased levels of the immunoregulator soluble ST2 (sST2) protein have been reported in the serum of patients with dengue, its importance during DENV infection remains unclear. The purpose of this study was to evaluate the effect of a recombinant human sST2 protein on the production of TNF- and IL-6 in an in vitro model of DENV infection. Peripheral blood mononuclear cells (PBMCs) were permissive to in vitro DENV infection since viral antigen was detected in CD14+ monocytes by flow cytometry (median, 1%; range, 0–2.2), and in their supernatants TNF- and IL-6 were detected. However, sST2 protein was not detected. Using multiple staining on infected PBMC we found that only CD14+ cells produced TNF- and IL-6. Treatment with human recombinant sST2 protein decreased lipopolysaccharide-induced monocyte TNF- and IL-6 production. However, this effect was not observed when the monocytes were pretreated with sST2 and later infected with DENV-2. These results suggest that sST2 has different roles in the regulation of TNF- and IL-6 expression in human monocytes stimulated with LPS and DENV-2.