Table of Contents
ISRN Biotechnology
Volume 2013, Article ID 236062, 6 pages
Research Article

Development of Biological Oxygen Demand Biosensor for Monitoring the Fermentation Industry Effluent

Department of Biotechnology, Punjabi University Patiala, Punjab, Patiala 147 002, India

Received 3 October 2012; Accepted 19 October 2012

Academic Editors: H. S. Garcia, S. Sanyal, and H. Stamatis

Copyright © 2013 Neelam Verma and Ashish Kumar Singh. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A biosensor was developed for the determination of BOD value of fermentation industry effluent. The developed biosensor was fabricated by immobilizing the microbial consortium on cellulose acetate (CA) membrane in close proximity to a DO probe electrode. The microbial consortium was harvested from the fermentation industry effluent. The BOD biosensor was calibrated by using a solution containing the equivalent amount of glucose/glutamic acid (GGA) as a standard sample solution. The response time was optimized by immobilizing different concentrations of cell biomass on CA membrane. Once the response time was optimized, it was used for determination of BOD of fermentation industry effluent. For analysis of fermentation industry effluent, the response time was observed 7 minutes with detection limit 1 mg/L. Good linear range with GGA standard solution was observed, 0.99 with relative standard deviation (RSD) <%. The observed BOD value by biosensor showed a good comparison with the conventional method for the determination of BOD.