The Role of Neurotransmitters in Protection against Amyloid-β Toxicity by KiSS-1 Overexpression in SH-SY5Y Neurons
Figure 12
Effect of endogenous enzyme inhibition on KiSS-1 gene overexpression neuroprotection against amyloid-β toxicity. PKiSS cells were pretreated with (a) catalase inhibitor 3-Amino-1,2,4-triazole (3AT: 50 mM), (b) cyclooxygenase inhibitor SC560 (1 μM), (c) nitric oxide synthase inhibitor NG-Methyl-L-arginine acetate salt (LNMA: 1 mM), (d) mitogen activated protein kinase cascade inhibitor PD98059 (50 μM) for 2 h prior to exposure to Aβ 1–40 (10 μM) and determination of viability by MTT reduction. Results are mean ± s.e.m. * versus control (media alone); † versus Aβ alone; one-way ANOVA.
