The Role of Neurotransmitters in Protection against Amyloid-β Toxicity by KiSS-1 Overexpression in SH-SY5Y Neurons

Figure 2

Effect of KiSS-1 gene overexpression on amyloid-β toxicity. (a) Human SH-SY5Y neurons stable cell lines containing control vector (PVect) or the KiSS-1 gene vector (PKiSS) were exposed to fibrillar Aβ 25–35 (10 μM) and cell viability determined by trypan blue exclusion. PKiSS cells were pretreated with (b) anti-kisspeptin antibody (Anti-KP: 10 μg/mL) or (c) kisspeptin receptor antagonist (KP234: 10 μM) or (d) neuropeptide FF receptor antagonist (RF9: 10 μM) for 2 h prior to exposure to fibrillar Aβ 1–40 (10 μM) and determination of viability by MTT reduction. Results are mean ± s.e.m. (a) ^§𝑃<0.05 versus PVect; **𝑃<0.05 versus PKiSS; ^§§𝑃<0.05 versus PVect + Aβ; (b–d) *𝑃<0.05 versus control (media alone); ^†𝑃<0.05 versus Aβ alone; one-way ANOVA.

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