Monocyte Migration Driven by Galectin-3 Occurs through Distinct Mechanisms Involving Selective Interactions with the Extracellular Matrix
Figure 2
Soluble or immobilized galectin-3 induces monocyte migration in a dose-dependent manner. In transwell systems, human monocytes ( cells) were added into the inserts and were allowed to migrate in response to different concentrations (0.001–0.9 M) of soluble (a) or immobilized (b) hrGal-3 for 40 min at 37°C. The number of migrating monocytes was counted in five fields for each condition assayed in triplicate. MCP-1 (100 ng/mL) was used as positive control and medium as negative. Data are reported as mean of monocytes per field SEM sGal-3, soluble Gal-3; iGal-3, immobilized Gal-3. * and ** compared to negative control. Results are representative from three independent assays.
