Monocyte Migration Driven by Galectin-3 Occurs through Distinct Mechanisms Involving Selective Interactions with the Extracellular Matrix
Figure 4
Galectin-3 binds to laminin through its carbohydrate recognition domain. A 96-well microplate was coated with 1µg/well of laminin or fibronectin and incubated for 2 h with biotinylated hrGal-3 or truncated Gal-3 (25 µg/mL). In some assays, biotinylated hrGal-3 was previously incubated with 10 mM lactose or sucrose. The signal was generated by streptavidin-peroxidase using 3,3′,5,5′-tetramethylbenzidine as substrate. Gal-3, galectin-3; TGal-3, truncated galectin-3; Lam, laminin; Fibr, fibronectin, Lac, lactose; Suc, sucrose. *** compared to Gal-3 in the absence of sugar. Results are representative from two independent assays.
