Table of Contents
ISRN Stem Cells
Volume 2013, Article ID 262451, 9 pages
http://dx.doi.org/10.1155/2013/262451
Research Article

Characterization of Bone-Marrow-Derived Stem Cells in Osteoporotic Models of the Rat

1Institute of Veterinary-Anatomy, -Histology and -Embryology of the Justus-Liebig-University Giessen, Frankfurter Straße 98, 35392 Giessen, Germany
2Clinic for Small Animals of the Justus-Liebig-University Giessen, Clinical Anatomy and Experimental Surgery, c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Frankfurter Straße 98, 35392 Giessen, Germany
3Department of Veterinary Clinical Sciences of the Justus-Liebig-University Giessen, Pathophysiology and Clinical Pathology, c/o Clinic for Small Animals, Frankfurter Straße 126, 35392 Giessen, Germany
4University Hospital of Giessen-Marburg, Department of Trauma Surgery, Rudolf-Buchheim-Straße 7, 35385 Giessen, Germany
5Medical Faculty of the Technical University Dresden, Institute of Physiological Chemistry, Fiedlerstraße 42, 01307 Dresden, Germany

Received 24 May 2013; Accepted 17 June 2013

Academic Editors: A. Chapel, F. Fagioli, and S. M. Hwang

Copyright © 2013 Julia Goergen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Osteoporotic effects observed after osteoporosis induction in the rat by combining ovariectomy (OVX) either with a defined calcium-deficient diet (OVX + Diet) or by administration of a glucocorticoid (dexamethasone) (OVX + Steroid) mimic the skeletal effects observed in humans affected by osteoporosis. In the present investigation rat MSCs have been characterized in vitro after osteoporosis has been induced for twelve weeks in rats by means of OVX + Diet () and OVX + Steroid (). Sham-operated animals () served as controls. MSCs were harvested from humerus and iliac crest and were cultured in standard medium and in osteogenic differentiation medium for studying the proliferation, migration, and differentiation capacity of the cells. Expression of CD90, CD105, runx2, osteocalcin (OC), and bone sialoprotein (BSP) was performed by using qrtPCR. Calcium deposits developed in the course of osteogenic differentiation were measured by using Pentra 400 Axon Lab. Taken together, the present results showed that osteoporosis induction leads to MSC in a state of senescence: proliferation and migration rates of the cells were diminished pointing to self-renewal deficiency and impaired motility of rat MSC in contrast to controls. However, the osteogenic differentiation capacity was increased after osteoporosis induction with OVX + Diet and OVX + Steroid.