Table of Contents
ISRN Pharmacology
Volume 2013, Article ID 271578, 11 pages
http://dx.doi.org/10.1155/2013/271578
Research Article

The Cytology, Isozyme, HPLC Fingerprint, and Interspecific Hybridization Studies of Genus Epimedium (Berberidaceae)

1School of Vocation and Technology, Guizhou Normal University, Guiyang, Guizhou 550001, China
2Institute of South China Karst, Guizhou Normal University, Guiyang 550001, China
3State Key Laboratory Incubation Base for Karst Mountain Ecology Environment of Guizhou Province, Guiyang 550001, China
4School of Life Science, Nanjing University, Nanjing, Jiangsu 210093, China

Received 23 May 2013; Accepted 1 August 2013

Academic Editors: G. Biala, G. M. Campo, R. Fantozzi, and D. K. Miller

Copyright © 2013 Lin-Jiao Wang and Mao-Yin Sheng. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.