Table of Contents
ISRN Chromatography
Volume 2013, Article ID 283462, 11 pages
Research Article

Profiling of Phytochemicals in Tissues from Sclerocarya birrea by HPLC-MS and Their Link with Antioxidant Activity

1Department of Food Biosciences, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland
2Department of Biology, University of Basilicata, V.le dell'Ateneo Lucano, 85100 Potenza, Italy
3Faculte des Sciences et Techniques, Universite Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal
4School of Agriculture and Food Science, University College Dublin, Dublin 4, Ireland

Received 27 March 2013; Accepted 29 April 2013

Academic Editors: R. F. D. Nascimento, T. Oi, Y. Qiu, and Y. Uesawa

Copyright © 2013 Daniela Russo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to investigate the differences in phytochemicals in roots, bark, and leaf of Sclerocarya birrea (marula) for methanol and water extracts that exhibited the best antioxidant activities. As many as 36 compounds were observed in the extracts of these tissues of which 27 phenolic compounds were tentatively identified. The HPLC-MS/MS results showed flavonoid glycosides were prominent in leaf extracts while the galloylated tannins were largely in bark and root extracts. Four flavonoid glycosides that were reported for the first time in the marula leaf have been identified. The HPLC-MS/MS studies also illustrated different degrees (highest degree = 3) of oligomerisation and galloylation of tannins in the bark and root extracts.