Table of Contents
ISRN Pharmaceutics
Volume 2013, Article ID 293935, 10 pages
Research Article

Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

1Department of Biochemistry, University College, Kurukshetra University, Kurukshetra, Haryana 136119, India
2Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana 124001, India
3National Research Institute of Basic Ayurvedic Sciences, Central Council for Research in Ayurvedic Sciences, Nehru Garden, Kothrud, Pune, Maharashtra 411038, India

Received 15 May 2013; Accepted 16 June 2013

Academic Editors: R. Cao, R. Lesyk, J. Peng, and S. Raic-Malic

Copyright © 2013 Ashwani Mittal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


High performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer was used for separation and identification of phenolic and other compounds in the water extracts of Saraca asoca (Roxb.), De. Wilde. The aim of the study was to identify and evaluate the distribution of phenolic compounds in the different parts of the plant. The identity of compounds was established through the comparison with standards and characteristic base peaks as well as other daughter ions. In crude extracts, 34 catechin derivatives, 34 flavonoids, and 17 other compounds were identified. Interestingly, further analysis of compounds showed plant part specific unique pattern of metabolites; that is, regenerated bark is observed to be the best source for catechin/catechin derivative while flowers were found to be the source for wide variety of flavonoids. Moreover, these plant part specific compounds can be used as biomarkers for the identification of plant material or herbal drugs. Overall, the present study provides for the first time a comprehensive analysis of the phenolic components of this herb which may be helpful not only to understand their usage but also to contribute to quality control as well.