Nontargeted Identification of the Phenolic and Other Compounds of Saraca asoca by High Performance Liquid Chromatography-Positive Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

Table 2

Identified polyphenols and their glycosides in different parts of S. asoca.

S. no.	RT	Name of compound	Product ions (m/z)	Calculated mass	Exact mass	Sample*
1	11.96	Kaempferol	112.01, 147.03, 163.134, 211.224, and 243.232	286.264	286.240	L
2	15.18	Kaempferol 3-diglucoside-7-glucoside-p-coumaloyl	471.167	918.198^#	918.191	B, F, RB
3	15.43	C-Hexosyl-apigenin	283.125, 367.723	528.528	528.520	F
4	21.40	Quercetin-3-rhamnoside	129.01, 141.025, 233.177, 287.147, 303.281, and 449.214	448.218	—	F, L
5	22.10	Petunidin-3-O-beta-glucopyranoside	317.125	479.122^#	479.118	F
6	22.33	Unknown gingerol type glycoside	139.123, 181.143, 265.213, 33.242, and 351.273	512.251^#	—	L
7	22.59	Pentahydroxyflavone-O-glucoside	129.12, 137.153, 153.123, and 305.833	466.265^#	466.157	F, L
8	22.72	C-Hexosyl-luteolin O-hexoside, O-pentoside	299.213, 329.142, 353.125, and 383.217	743.286	742.278	FW
9	23.20	Peonidin-3-O-𝛽-galactopyranoside	301.145	463.184^#	463.124	F, L
10	23.57	Dihexosyl quercetin	303.124, 465.213	626.134	626.150	F
11	23.78	Quercetin	123.10, 137.24, 151.33, and 285.77	302.796	302.04265	B, RB
12	24.10	Quercetin-3′,7-di-O-glucoside	287.249, 449.364	610.411	610.52	F
13	24.14	Isorhamnetin sophorose	317.263, 479.223	640.167^#	640.160	F
14	24.52	6-Hydroxykaempferol	123.102, 139.214, 147.31, 151.3358, 165.43, 181.518, 193.57, 207.63, 215.668, 243.722, 261.67, and 285.785	302.796	302.042	B
15	24.52	C-Hexosyl-chrysoeriol O-hexoside	301.124, 463.256	624.204^#	624.17	F
16	24.70	3,5,7,2′,6′-Pentahydroxyflavone	215.727, 243.82, and 289.811	306.864^#	304.058	L
17	24.77	Malvidin-3-galactoside	331.772	493.112^#	493.134	F
18	24.80	Peonidin glucoside derivative	301.767, 463.654	776.145^#	—	F
19	24.88	Peonidin-3,5-O-di-𝛽-glucopyranoside	286.0235, 301.0235	625.241^#	625.176	F
20	24.90	Phloridzin^#	275.421	437.542^#	436.136	RB
21	24.99	Peonidin-3-O-alpha-arabinopyranoside	133.023, 177.253, 301.271, and 415.123	433.105^#	433.113	F
22	25.03	(+)-Dihydrokaempferol	107.04, 123.09, 127.13, 139.23, 149.29, 163.407, 166.472, 179.434, 215.654, 243.715, 259.759, 271.744, and 289.763	288.763	288.063	ALL
23	25.08	3-O-Hexosyl-quercetin	133.197, 145.295, 153.356, and 301.178	464.075	464.10	F
24	25.22	Leucopelargonidin 3-O-glucoside	137.124, 291.178, and 303.155	452.185	452.131	ALL
25	25.26	Apigenin	107.043, 119.003, 149.013, 153.123, 174.155, 215.165, 228.213, and 243.214	270.103	270.05	ALL
26	25.43	Malvidin-diglucoside	331.275, 493.213	655.257^#	655.187	F, L
27	25.46	Isorhamnetin-3-coumaroylglucopyranoside	317.275, 463.266	624.224^#	—	B
28	25.70	Isorhamnetin	115.043, 123.176, 147.109, 165.133, 257.177, 297.020, and 302.054	316.02^#	316.06	B
29	26.5	Delphinidin-3-O-𝛽-glucopyranoside	303.213	465.231^#	465.103	F
30	27.28	Quercetin-3-O-Arabinoside	131.01, 137.024, 151.125, 181.123, 257.263, 285.214, 303.214, and 360.225	434.2	434.214	B
31	27.43	Quercetin 3,4′-di-glucoside-3′-(6-caffeoylglucoside)	625, 787, and 487.685	950.265	950.257	RB
32	30.20	7-Acetyloxy-2-methylisoflavone	107.08, 111.077, 121.134, 125.068, 151.100, 161.187, 179.194, 193.170, 221.191, 237.146, 249.178, 259.176, and 277.103	294.102^#	294.089	F
33	30.47	Peonidin	286.0235, 301.068	301.067^#	301.071	ALL
34	34.60	Isorhamnetin-3-O-glucoside	302.155, 317.013	478.032^#	478.111	F

∗Abbreviations B, F, L and RB in sample column represent bark, flower, leaves, and regenerated bark, respectively.
^#Compound detected for the first time in S. asoca.