Table of Contents
ISRN Parasitology
Volume 2013, Article ID 308726, 5 pages
Research Article

Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis

1Department of Animal Biology, Faculty of Science, Damascus University, Damascus 96311, Syria
2Vector Biology Research Program, United States Naval Medical Research Unit No. 3 (NAMRU-3), Cairo 11517, Egypt

Received 25 June 2013; Accepted 13 August 2013

Academic Editors: L. Nimri, O. F. Osman, J. M. Perez, and G. Swedberg

Copyright © 2013 Samar Anis Al-Nahhas and Rania Magdy Kaldas. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cutaneous leishmaniasis (CL) is an endemic disease and a public health problem in Hama governorate located in the central region of Syria. The aim of this study was to characterize Leishmania species isolated from human skin samples. A polymerase chain reaction, restriction fragment length polymorphism (PCR-RFLP) assay, was performed on skin lesion material samples from 32 patients with confirmed CL by direct microscopic examination in order to prove its usefulness and efficiency for identification of Leishmania species. Leishmania tropica (L. tropica) is confirmed as an etiologic agent of CL in this area.