Acute Activation of the Renal Betaine/GABA Transporter in Response to a Decrease in Extracellular Calcium

Figure 3

Additional activation of BGT1 transport in plastic-grown MDCK monolayers by acute exposure to low Ca²⁺ hypertonic growth medium following overnight (16 h) adaptation to normal hypertonic medium. (a) Na⁺-dependent betaine transport was significantly activated after 60 min in low Ca²⁺ medium compared to normal hypertonic controls. (b) Activation of Na⁺-dependent GABA transport by 60 min exposure to hypertonic low Ca²⁺ medium was reversed completely within 2 h after return to normal hypertonic growth medium. *Significantly different (𝑃<0.05) compared to Hyp controls. Data are mean ± SD from 5 separate experiments.

(a)

(b)