Table of Contents
ISRN Agronomy
Volume 2013, Article ID 317903, 12 pages
Research Article

Effects of Media Formulation on the Growth and Morphology of Ectomycorrhizae and Their Association with Host Plant

Department of Agro-Environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 811-2415, Japan

Received 18 January 2013; Accepted 6 February 2013

Academic Editors: A. D. Arencibia, J. S. Swanston, and I. Vasilakoglou

Copyright © 2013 Ferzana Islam and Shoji Ohga. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Tricholoma matsutake and Rhizopogon roseolus form ectomycorrhizal (ECM) association with their host plant on natural habitats. The main objective of this study was to test mycelial growth, morphology, and host plant survival both in vitro and in vivo when treated with enriched media. Aseptically germinated seedlings of Pinus densiflora and P. thunbergii were inoculated with the strains of T. matsutake and R. roseolus, respectively. Under in vitro conditions mycelial growth rates performed best on pH 5 and were better on Modified-Melin-Norkrans-(MMN) based medium and Potato Dextrose Agar (PDA); addition of micronutrients and vitamins in MMN mycelial growth rates had 6–27% differences. Without ECM, plant survival rates on standard media were 30% to below 30% and by inclusion of elements they were 50% to 80%. On in vivo, soil containing different media with ECM allowed successful mycorrhizal association and increased seedling survival rates approximately 100%. Our findings confirm that MMN and PDA allowed higher mycelial growth but poor plant survival (<30%); however, enriched media supported 100% plant survival with successful ECM associations. The present method is advantageous in terms of giving objectivity for ECM by employing suitable media for strains and host plant, and making it possible for mass production of ECM-infected seedlings.