Table of Contents
ISRN Veterinary Science
Volume 2013, Article ID 323671, 4 pages
http://dx.doi.org/10.1155/2013/323671
Research Article

Detection of Mycobacterium avium Subspecies Paratuberculosis from Intestinal and Nodal Tissue of Dogs and Cats

Departments of Clinical Sciences (KuKanich) and Diagnostic Medicine/Pathobiology (Vinasco, Scott), Q-213 Mosier Hall, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA

Received 2 August 2013; Accepted 25 August 2013

Academic Editors: B. China and H. Fukushi

Copyright © 2013 Kate S. KuKanich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To determine prevalence of MAP in intestinal and nodal tissue from dogs and cats at necropsy at Kansas State University and to determine if an association existed between presence of MAP and gastrointestinal inflammation, clinical signs, or rural exposure. Procedures. Tissue samples were collected from the duodenum, ileum, and mesenteric and colic nodes of adult dogs (73) and cats (37) undergoing necropsy for various reasons. DNA was extracted and analyzed for insertion sequence 900 using nested PCR. Positive samples were confirmed with DNA sequencing. An online mapping system was used to determine if patients lived in an urban or rural environment based on the home address. Medical records were reviewed for clinical signs and histological findings at necropsy. Results. MAP was identified from 3/73 (4.1%) dogs and 3/37 (8.1%) cats. There was no documented association between presence of MAP and identification of histologic-confirmed gastrointestinal inflammation, gastrointestinal clinical signs, or exposure to a rural environment. Conclusion and Clinical Relevance. MAP-specific DNA can be identified within the intestinal and nodal tissue of dogs and cats that do not have pathological lesions or clinical signs consistent with gastrointestinal disease. The significance of this organism’s presence without associated gastrointestinal pathology is unknown.