Research Article
Cefpodoxime Proxetil: A New Stability Indicating RP-HPLC Method
Table 5
Robustness evaluation.a
| | Factor | Level | Retention time (Rt) for both isomers of CP (min) | Total area for both isomers |
| | (A) Flow rate (mL min−1) | | 0.95 | −1 | 7.212 | 8.122 | 5280908 | | 1 | 0 | 7.066 | 7.927 | 5239656 | | 1.05 | +1 | 6.99 | 7.836 | 5272832 | | Mean ± SD ( = 3) | 7.089 ± 0.113 | 7.962 ± 0.146 | 5264465 ± 21861 |
| | (B) Percentage of methanol in mobile phase | | 64 | −1 | 7.133 | 8.01 | 5339448 | | 65 | 0 | 7.066 | 7.927 | 5239656 | | 66 | +1 | 6.911 | 7.82 | 5311649 | | Mean ± SD (n = 3) | 7.037 ± 0.114 | 7.934 ± 0.099 | 5296917.67 ± 51501 |
| | (C) Wavelength of measurement | | 251 | −1 | 7.068 | 7.932 | 5256020 | | 252 | 0 | 7.066 | 7.927 | 5239656 | | 253 | +1 | 7.095 | 7.912 | 5369302 | | Mean ± SD ( = 3) | 7.0763 ± 0.016 | 7.924 ± 0.010 | 5288326 ± 70603 |
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aMean values of six trials.
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