Table of Contents
ISRN Microbiology
Volume 2013, Article ID 342082, 6 pages
http://dx.doi.org/10.1155/2013/342082
Research Article

16S rRNA PCR-Denaturing Gradient Gel Electrophoresis of Oral Lactobacillus casei Group and Their Phenotypic Appearances

1Common Oral Diseases and Epidemiology Research Center, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand
2Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand
3Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand

Received 29 June 2013; Accepted 25 August 2013

Academic Editors: O. Pepe and A. Sunna

Copyright © 2013 S. Piwat and R. Teanpaisan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. H. Meurman and I. Stamatova, “Probiotics: contributions to oral health,” Oral Diseases, vol. 13, no. 5, pp. 443–451, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. S. Salminen, A. C. Ouwehand, and E. Isolauri, “Clinical applications of probiotic bacteria,” International Dairy Journal, vol. 8, no. 5-6, pp. 563–572, 1998. View at Publisher · View at Google Scholar · View at Scopus
  3. S. Parvez, K. A. Malik, S. Ah Kang, and H.-Y. Kim, “Probiotics and their fermented food products are beneficial for health,” Journal of Applied Microbiology, vol. 100, no. 6, pp. 1171–1185, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. R. Hemalatha, P. Mastromarino, B. A. Ramalaxmi, N. V. Balakrishna, and B. Sesikeran, “Effectiveness of vaginal tablets containing lactobacilli versus pH tablets on vaginal health and inflammatory cytokines: a randomized, double-blind study,” European Journal of Clinical Microbiology and Infectious Diseases, vol. 31, no. 11, pp. 3097–3105, 2012. View at Google Scholar
  5. M. D. Collins, U. Rodrigues, C. Ash et al., “Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA,” FEMS Microbiology Letters, vol. 77, no. 1, pp. 5–12, 1991. View at Publisher · View at Google Scholar · View at Scopus
  6. G. E. Felis and F. Dellaglio, “Taxonomy of lactobacilli and bifidobacteria,” Current Issues in Intestinal Microbiology, vol. 8, no. 2, pp. 44–61, 2007. View at Google Scholar · View at Scopus
  7. F. Chavagnat, M. Haueter, J. Jimeno, and M. G. Casey, “Comparison of partial tuf gene sequences for the identification of lactobacilli,” FEMS Microbiology Letters, vol. 217, no. 2, pp. 177–183, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. C. M. Dobson, B. Chaban, H. Deneer, and B. Ziola, “Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus zeae isolates identified by sequence signature and immunoblot phenotype,” Canadian Journal of Microbiology, vol. 50, no. 7, pp. 482–488, 2004. View at Publisher · View at Google Scholar · View at Scopus
  9. A. Vásquez, S. Ahrné, B. Pettersson, and G. Molin, “Temporal temperature gradient gel electrophoresis (TTGE) as a tool for identification of Lactobacillus casei, Lactobacillus paracasei, Lactobacillus zeae and Lactobacillus rhamnosus,” Letters in Applied Microbiology, vol. 32, no. 4, pp. 215–219, 2001. View at Publisher · View at Google Scholar · View at Scopus
  10. J. Walter, G. W. Tannock, A. Tilsala-Timisjarvi et al., “Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers,” Applied and Environmental Microbiology, vol. 66, no. 1, pp. 297–303, 2000. View at Google Scholar · View at Scopus
  11. B. Biavati, “International Committee of Systematic Bacteriology. Subcommitee on the taxonomy of Bifidobacterium, Lactobacillus and related organisms. Minutes of meeting, 22 and 23 August 1996,” International Journal of Systematic and Evolutionary Microbiology, vol. 51, no. 6, pp. 257–258, 2001. View at Google Scholar
  12. G. Klein, “International Committee of Systematic Bacteriology. Subcommitee on the taxonomy of Bifidobacterium, Lactobacillus and related organisms. Minutes of meeting, 22 and 23 September 1999,” International Journal of Systematic and Evolutionary Microbiology, vol. 51, no. 6, pp. 259–261, 2001. View at Google Scholar
  13. G. E. Felis, F. Dellaglio, L. Mizzi, and S. Torriani, “Comparative sequence analysis of a recA gene fragment brings new evidence for a change in the taxonomy of the Lactobacillus casei group,” International Journal of Systematic and Evolutionary Microbiology, vol. 51, no. 6, pp. 2113–2117, 2001. View at Google Scholar · View at Scopus
  14. R. Teanpaisan and G. Dahlén, “Use of polymerase chain reaction techniques and sodium dodecyl sulfate-polyacrylamide gel electrophoresis for differentiation of oral Lactobacillus species,” Oral Microbiology and Immunology, vol. 21, no. 2, pp. 79–83, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. R. Teanpaisan, S. Piwat, and G. Dahlén, “Inhibitory effect of oral Lactobacillus against oral pathogens,” Letters in Applied Microbiology, vol. 53, no. 4, pp. 452–459, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. S. Piwat, R. Teanpaisan, G. Dahlén, S. Thitasomakul, and C. W. Douglas, “Acid production and growth by oral Lactobacillus species in vitro,” Journal of Investigative and Clinical Dentistry, vol. 3, no. 1, pp. 56–61, 2012. View at Google Scholar
  17. S. Santosa, E. Farnworth, and P. J. H. Jones, “Probiotics and their potential health claims,” Nutrition Reviews, vol. 64, no. 6, pp. 265–274, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. M. D. Collins, B. A. Phillips, and P. Zanoni, “Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and Lactobacillus rhamnosus sp. nov., comb. nov,” International Journal of Systematic Bacteriology, vol. 39, no. 2, pp. 105–108, 1989. View at Google Scholar · View at Scopus
  19. H. Chen, C. K. Lim, Y. K. Lee, and Y. N. Chan, “Comparative analysis of the genes encoding 23S-5S rRNA intergenic spacer regions of Lactobacillus casei-related strains,” International Journal of Systematic and Evolutionary Microbiology, vol. 50, no. 2, pp. 471–478, 2000. View at Google Scholar · View at Scopus
  20. F. Dellaglio, L. M. T. Dicks, M. Du Toit, and S. Torriani, “Designation of ATCC 334 in place of ATCC 393 (NCDO 161) as the neotype strain of Lactobacillus casei subsp. casei and rejection of the name Lactobacillus paracasei,” International Journal of Systematic Bacteriology, vol. 41, no. 2, pp. 340–342, 1991. View at Google Scholar · View at Scopus
  21. F. Dellaglio, G. E. Felis, and S. Torriani, “The status of the species Lactobacillus casei (Orla-Jensen 1916) Hansen and lessel 1971 and Lactobacillus paracasei Collins et al. 1989. Request for an opinion,” International Journal of Systematic and Evolutionary Microbiology, vol. 52, no. 1, pp. 285–287, 2002. View at Google Scholar · View at Scopus
  22. A. Vásquez, G. Molin, B. Pettersson, M. Antonsson, and S. Ahrné, “DNA-based classification and sequence heterogeneities in the 16S rRNA genes of Lactobacillus casei/paracasei and related species,” Systematic and Applied Microbiology, vol. 28, no. 5, pp. 430–441, 2005. View at Publisher · View at Google Scholar · View at Scopus
  23. B. Ayna, S. Çelenk, F. Atakul, B. Sezgin, and T. Özekinci, “Evaluation of clinical and microbiological features of deep carious lesions in primary molars,” Journal of Dentistry for Children, vol. 70, no. 1, pp. 15–18, 2003. View at Google Scholar · View at Scopus
  24. L. Bjørndal and T. Larsen, “Change in the cultivable flora in deep carious lesion following a stepwise excavation procedure,” Caries Research, vol. 34, no. 6, pp. 502–508, 2000. View at Google Scholar · View at Scopus
  25. M.-S. Kang, J.-S. Oh, H.-C. Lee et al., “Inhibitory effect of Lactobacillus reuteri on periodontopathic and cariogenic bacteria,” Journal of Microbiology, vol. 49, no. 2, pp. 193–199, 2011. View at Publisher · View at Google Scholar · View at Scopus
  26. K. Hatakka, A. J. Ahola, H. Yli-Knuuttila et al., “Probiotics reduce the prevalence of oral Candida in the elderly—a randomized controlled trial,” Journal of Dental Research, vol. 86, no. 2, pp. 125–130, 2007. View at Publisher · View at Google Scholar · View at Scopus
  27. M. Matsumoto, M. Tsuji, H. Sasaki et al., “Cariogenicity of the probiotic bacterium Lactobacillus salivarius in rats,” Caries Research, vol. 39, no. 6, pp. 479–483, 2005. View at Publisher · View at Google Scholar · View at Scopus
  28. R. Byun, M. A. Nadkarni, K.-L. Chhour, F. E. Martin, N. A. Jacques, and N. Hunter, “Quantitative analysis of diverse Lactobacillus species present in advanced dental caries,” Journal of Clinical Microbiology, vol. 42, no. 7, pp. 3128–3136, 2004. View at Publisher · View at Google Scholar · View at Scopus
  29. S. I. Smith, A. J. Aweh, A. O. Coker, K. O. Savage, D. A. Abosede, and K. S. Oyedeji, “Lactobacilli in human dental caries and saliva,” Microbios, vol. 105, no. 411, pp. 77–85, 2001. View at Google Scholar · View at Scopus
  30. S. Nagaoka, H.-J. Liu, K. Minemoto, and M. Kawagoe, “Microbial induction of dentinal caries in human teeth in vitro,” Journal of Endodontics, vol. 21, no. 11, pp. 546–551, 1995. View at Publisher · View at Google Scholar · View at Scopus