Stability-Indicating Validated Novel RP-HPLC Method for Simultaneous Estimation of Methylparaben, Ketoconazole, and Mometasone Furoate in Topical Pharmaceutical Dosage Formulation

Table 2

Results of forced degradation study for MP, KT, and MF.

Stress conditions	MP			KT			MF
	PA	PTH	% Deg.	PA	PTH	% Deg.	PA	PTH	% Deg.
Acidic hydrolysis (5 N HCl, 70°C, 30 mins)	0.319	1.105	8.1	0.126	1.181	54.1	0.655	1.950	ND
Alkaline hydrolysis (0.05 N NaOH, RT, 15 mins)	0.286	1.113	ND	0.173	1.141	ND	1.181	2.057	11.3
Oxidation (30% H2O2, RT, 45 min)	0.094	1.127	ND	0.204	1.159	12.4	0.760	2.029	ND
Thermal (At 80°C, 6 h)	0.165	1.179	ND	0.177	1.143	ND	1.286	2.216	ND
UV and visible light exposed	0.286	1.113	4.2	0.173	1.141	17.8%	1.181	2.057	ND

N.D.: no degradation, RT: room temperature, PA: purity angle, PTH: purity threshold.