Table of Contents
ISRN Bioinformatics
Volume 2013 (2013), Article ID 361321, 8 pages
http://dx.doi.org/10.1155/2013/361321
Research Article

Transcriptome Analysis of Spermophilus lateralis and Spermophilus tridecemlineatus Liver Does Not Suggest the Presence of Spermophilus-Liver-Specific Reference Genes

1Raffles Institution, One Raffles Institution Lane, 575954, Singapore
2Department of Zoology, The University of Melbourne, Genetics Lane, Parkville, VIC 3010, Australia
3Department of Mathematics and Statistics, South Dakota State University, SD 57007, USA

Received 25 March 2013; Accepted 23 April 2013

Academic Editors: A. Bolshoy and D. Labudde

Copyright © 2013 Bryan M. H. Keng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The expressions of reference genes used in gene expression studies are assumed to be stable under most circumstances. However, studies had demonstrated that genes assumed to be stably expressed in a species are not necessarily stably expressed in other organisms. This study aims to evaluate the likelihood of genus-specific reference genes for liver using comparable microarray datasets from Spermophilus lateralis and Spermophilus tridecemlineatus. The coefficient of variance (CV) of each probe was calculated and there were 178 probes common between the lowest 10% CV of both datasets ( ). All 3 lists were analysed by NormFinder. Our results suggest that the most invariant probe for S. tridecemlineatus was 02n12, while that for S. lateralis was 24j21. However, our results showed that Probes 02n12 and 24j21 are ranked 8644 and 926 in terms of invariancy for S. lateralis and S. tridecemlineatus respectively. This suggests the lack of common liver-specific reference probes for both S. lateralis and S. tridecemlineatus. Given that S. lateralis and S. tridecemlineatus are closely related species and the datasets are comparable, our results do not support the presence of genus-specific reference genes.