Quantification of the Spatial Organization of the Nuclear Lamina as a Tool for Cell Classification
Figure 2
LMNA accumulation on the nuclear envelop is enriched in senescent cells. (a) Images of representative nuclei show LMNA staining in WI38 fibroblasts at early and late passage number (passage 13 and 27, resp.). Cells were mock treated or treated with 1% triton prior to fixation. LMNA is visualized with mouse-antibodies in red, and the DNA is counterstained with DAPI. After triton treatment, heterochromatic foci are disrupted. LMNA fluorescent intensities over the lines through the nuclei are shown in the lower figures. (b) LMNA enrichment at the nuclear envelope. LMNA enrichment at the nuclear envelop is normalized to the LMNA signal across the nucleus. Means and standard deviations are shown for 100 nuclei.
