Development and Validation of High Performance Liquid Chromatographic Analysis of Residual N,N-Dimethylformamide in Spent Medium after Biodegradation by Paracoccus denitrificans SD1

Table 3

Accuracy results of DMF estimation method at different dilutions.

DMF (%)	Injections	Peak area	% Recovery	% RSD
0.25	Initial	3962929	99.12	0.26
	Half dilution	1982512	99.49
	Quaternary dilution	991319	98.97
0.50	Initial	8134734	98.97	0.15
	Half dilution of initial	3995677	99.15
	Quaternary dilution	2012345	99.27
0.75	Initial	11219687	99.14	0.19
	Half dilution of initial	5598767	99.43
	Quaternary dilution	2804845	99.06
1.0	Initial	13764192	99.24	0.17
	Half dilution of initial	68902871	98.98
	Quaternary dilution	3441065	99.32