Table of Contents
ISRN Infectious Diseases
Volume 2013, Article ID 420738, 7 pages
http://dx.doi.org/10.5402/2013/420738
Research Article

Nosocomial Pneumonia Associated to PVL-Producing Staphylococcus aureus in Children in Benin

1Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Département de Biochimie et de Biologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin
2Laboratoire de Biologie Humaine, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Benin
3Institut de Bactériologie, Université de Strasbourg, Unité: EA-4438 Physiopathologie et Médecine, 3 rue Koeberlé, 67000 Strasbourg, France
4Institut Régional de Santé Publique, Université d’Abomey-Calavi, BP 384 Ouidah, Benin
5Laboratoire de Pharmacologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Benin
6Department of Biology and Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, 315 Penn Street, Camden, NJ 08102, USA

Received 19 June 2013; Accepted 15 July 2013

Academic Editors: D. Bachani and A. Carvalho

Copyright © 2013 Lamine Baba-Moussa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We determined the type of toxins produced by Staphylococcus aureus strains and the possible source of contamination during an outbreak of nosocomial pneumonia in a Paediatric service in Benin. Data of 37 patients admitted in the malnourished unit who were diagnosed with pneumonia according to WHO definition and with radiological evidence of a pulmonary infiltrate were collected within six weeks. Pneumonia was further confirmed by the identification of corresponding pneumonia-related S. aureus. Samples were also collected from hospital personnel, the environment, and the food fed to the patients. Pulsed field gel electrophoresis (PFGE) was used to compare the bacterial profile from different sources. Among the 37 patients admitted during this period, 17 developed pneumonia and 10 were associated with S. aureus strains. Nine patients infected with S. aureus PVL-producing strains had underlying diseases and developed an acute multilobar pneumonia, which was fulminating and rapidly became fatal in all except the oldest child. Most of the isolates found on sick children were similar to those from special nutrients consumed by children and on the personnel at the hospital. The highly probable relationships between children contamination and isolate presence in the special nutrient underline the rapid and disastrous dissemination of some PVL-producing isolates in this paediatric unit.