Vitamin D Receptor Gene BsmI Polymorphism in Polish Patients with Systemic Lupus Erythematosus

Table 2

Real-time PCR reaction conditions.

Parameter	Program
	Denat.	Cycling			Melting			Cooling
Analysis mode	None	Quantification			Melting curves			None
Cycles	1	45			1			1
Segment	1	1	2	3	1	2	3	1
Target [°C]	95	95	60	72	95	40	75	40
Hold [hh:mm:ss]	00:10:00	00:00:10	00:00:10	00:00:15	00:00:30	00:02:00	00:00:00	00:00:30
Ramp rate [°C/s]	4.4	4.4	2.2	4.4	4.4	1.5	—	1.5
Acquisition mode	None	None	Single	None	None	None	Continu.	None
Acquisition per [°C]							3