Table of Contents
ISRN Spectroscopy
Volume 2013 (2013), Article ID 431801, 6 pages
http://dx.doi.org/10.1155/2013/431801
Research Article

Characterization of Arsenic Biotransformation Products from an Open Anaerobic Degradation of Fucus distichus by Hydride Generation Gas Chromatography Atomic Absorption Spectrometry and High Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry

Department of Chemical Sciences, Afe Babalola University, PMB, Ado Ekiti 5454, Nigeria

Received 30 August 2013; Accepted 23 September 2013

Academic Editors: J. H. Z. Dos Santos, C. S. Ha, C. Liang, and R. E. Santelli

Copyright © 2013 Abiodun A. Ojo and Amos Onasanya. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This work reports on the isolation and determination of biotransformation products obtained from the organoarsenic compounds that are present in Fucus distichus when it was subjected to an open anaerobic decomposition by using the Hydride Generation Gas Chromatography Atomic Absorption Spectrometry (HG-GC-AAS) and High Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP-MS). The seaweed and filtrate residues obtained from the open anaerobic degradation procedure were extracted in methanol and partitioned in phenol-ether-water mixtures to obtain water soluble extracts. The water soluble extracts were cleaned up and separated on a gel permeation Sephadex G15 column. Arsenic species concentrations were determined by using HG-GC-AAS. Final characterization of the biotransformation isolates was carried out on HPLC-ICP-MS. Only two arsenic species, 2-dimethylarsinoyl ethanol (DMAE) and dimethylarsinic acid (DMAA), were positively identified in the water soluble extract of the marine brown algae. The two arsenic species are strong intermediate candidates in the biosynthesis of arsenobetaine from oceanic arsenate in marine food webs.