Table of Contents
ISRN Biotechnology
Volume 2013, Article ID 453910, 5 pages
Research Article

A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides

1Institute of Quality Standards for Agriculture Products, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
2College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China
3Departmentof Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China

Received 12 May 2012; Accepted 24 July 2012

Academic Editors: H. S. Garcia and J. Jia

Copyright © 2013 Wei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9–3.3) and 30% B (100% acetonitrile) using an Isogradient program. The flow rate was 0.5 mL/min. The absorb wavelength was 226.5 nm; the column temperature was controlled at 25°C. This method for angiotensin I-converting enzyme inhibitory peptides assay was convenient for the Iso-gradient program. The accuracy of the RT-HPLC method was verified by analyzing ACE inhibitory activity of the hydrolysate peptides of silkworm pupae protein, and the results showed that the RT-HPLC method was available for exploring new source of angiotensin I-converting enzyme inhibitory peptides rapidly and veraciously.