Table of Contents
ISRN Bioinformatics
Volume 2013 (2013), Article ID 481545, 8 pages
http://dx.doi.org/10.1155/2013/481545
Research Article

Stormbow: A Cloud-Based Tool for Reads Mapping and Expression Quantification in Large-Scale RNA-Seq Studies

1Systems Pharmacology and Biomarkers, Janssen Research & Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, USA
2High Performance & Scientific Computing, Janssen Research & Development, LLC, 920 Route 202, Raritan, NJ 08869, USA
3Translational Informatics IT, Janssen Research & Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, USA

Received 8 July 2013; Accepted 7 August 2013

Academic Editors: N. Lemke, K. Mizuguchi, O. Norberto de Souza, and J. T. L. Wang

Copyright © 2013 Shanrong Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

RNA-Seq is becoming a promising replacement to microarrays in transcriptome profiling and differential gene expression study. Technical improvements have decreased sequencing costs and, as a result, the size and number of RNA-Seq datasets have increased rapidly. However, the increasing volume of data from large-scale RNA-Seq studies poses a practical challenge for data analysis in a local environment. To meet this challenge, we developed Stormbow, a cloud-based software package, to process large volumes of RNA-Seq data in parallel. The performance of Stormbow has been tested by practically applying it to analyse 178 RNA-Seq samples in the cloud. In our test, it took 6 to 8 hours to process an RNA-Seq sample with 100 million reads, and the average cost was $3.50 per sample. Utilizing Amazon Web Services as the infrastructure for Stormbow allows us to easily scale up to handle large datasets with on-demand computational resources. Stormbow is a scalable, cost effective, and open-source based tool for large-scale RNA-Seq data analysis. Stormbow can be freely downloaded and can be used out of box to process Illumina RNA-Seq datasets.