Table of Contents
ISRN Chromatography
Volume 2013, Article ID 484592, 7 pages
Research Article

Determination of Ranitidine in Human Plasma by SPE and ESI-LC-MS/MS for Use in Bioequivalence Studies

1Laboratório de Métodos de Extração e Separação (LAMES), Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, CP 131, 74001-970 Goiânia, GO, Brazil
2Núcleo Integrado de Farmacocinética da Universidade Federal de Goiás e Instituto Melon de Estudos e Pesquisas/Instituto de Ciências Farmacêuticas, CP 131, 74001-970 Goiânia, GO, Brazil

Received 9 November 2012; Accepted 16 December 2012

Academic Editors: J.-F. Jen, J. Millership, and A. Sanches Silva

Copyright © 2013 Karini B. Bellorio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A method for determining ranitidine in human plasma by ESI-LC-MS/MS was validated, using propranolol as internal standard. The extraction method used was solid phase extraction (SPE). Chromatographic separation was performed in a Chromolith C18 (50 mm × 4.6 mm i.d.) analytical column, which provided good separation of ranitidine and propranolol peaks with an analysis time of 2.5 minutes. Extraction yields of 94.4% for ranitidine and 89.4% for the internal standard were obtained. The lower limit of quantification (LLOQ) was 3.00 ng/mL, and limit of detection (LOD) was 0.05 ng/mL, with linearity ranging from 3.00 to 500 ng/mL. The results, thus, showed that this method is suitable for application in bioequivalence studies of ranitidine in human plasma.