Table of Contents
ISRN Hepatology
Volume 2013 (2013), Article ID 508368, 5 pages
http://dx.doi.org/10.1155/2013/508368
Research Article

Quantifying Serum Level of Glycochenodeoxycholic Acid Using High Performance Liquid Chromatography in Obstructive Jaundice Patients

1Digestive Surgery Division, Cipto Mangunkusumo Hospital and Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia
2Department of Surgery, Faculty of Medicine, University of Indonesia, Medical Staff Building 4th Floor, Jalan Diponegoro No. 71, Jakarta 10430, Indonesia
3Department of Pharmacology & Therapeutic, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia

Received 12 June 2012; Accepted 25 July 2012

Academic Editors: C. Domenicotti, A. Maieron, and A. Nanashima

Copyright © 2013 Toar J. M. Lalisang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Accumulation of glycochenodeoxycholic acid (GCDC) in serum has a clinical significance as an inductor of pathological hepatocyte apoptosis, which impairs liver function. Inhibition of GCDC accumulation can be used as a marker in therapy. This study was aimed to quantify the serum level of GCDC in obstructive jaundice patients. Methodology. GCDC acid level in the serum was quantified using high performance liquid chromatography (HPLC) technique according to Muraca and Ghoos modified method. It was performed before and after decompression at day 7 and day 14. The sample was extracted with solid phase extraction (SPE) technique on SPE column. The results were analyzed using SPSS V 16.0 ( ) and quantified with standard curve on GCDC acid. Result. There were 21 cases with range of GCDC acid serum level before decompression was 90.9 (SD 205.5) μmol/L and day 7 after decompression decreased to 4.0 (SD 46.4) μmol/L and then increased to 11.3 (SD 21.9) μmol/L ( ). This method could separate GCDC acid on serum with good resolution, high precision and accuracy, and linear calibration curve on measured level range. Conclusion. HPLC can quantify GCDC acid serum on obstructive jaundice patients and can be used to support its pharmacokinetic study.