Identification of T- and B-Cell Subsets That Expand in the Central and Peripheral Lymphoid Organs during the Establishment of Nut Allergy in an Adjuvant-Free Mouse Model
Figure 2
Gating strategy used to identify T- and B-lymphocyte subsets. Shown is one representative spleen sample. (a) Gating of memory T cells. Based on forward scatter, and side scatter live cells were gated and a histogram of CD3 (a(i)) was used to gate CD3+ cells. Subsequently, CD3+ cells were separated into CD4+ and CD8+ cells based on dot plot (a(ii)). Central memory T cells were gated as CD62Lhi/CD44hi and effector memory T cells were gated as CD62Lneg/CD44hi out of both CD4+ (a(iv)) and CD8+ T cells (a(iii)). (b) Gating of memory B cells. Based on forward scatter and side scatter, live cells were gated and a histogram of B220 (b(i)) was used to gate B220+ cells. In the bone marrow, only B220hi cells were gated (data not shown). Subsequently, B cells were gated in the dot plot based on CD62L and CD80 expression (b(ii)). The expression of CD73 was assessed by histogram (b(iii)); only CD80+/CD62Lneg quadrant is shown as a representative example.
