Identification of T- and B-Cell Subsets That Expand in the Central and Peripheral Lymphoid Organs during the Establishment of Nut Allergy in an Adjuvant-Free Mouse Model
Figure 3
((a)–(c)) Analysis of central and peripheral phenotype of T lymphocytes from hazelnut allergic and nonallergic control mice. Groups of BALB/c ( to 7 per group) were sensitized with hazelnut protein (0.5 mg/mouse) via transdermal exposure and then confirmed for allergy by oral challenge. After 1 hour, bone marrow (a), spleen (b), and mesenteric lymph nodes (MLN) (c) were harvested and single-cell suspension prepared. Cells were stained with antibodies against CD3, CD4, and CD62L markers and analyzed by flow cytometry. Differences between allergic and nonallergic mice data were compared by Student’s -test for significance ().
