Identification of T- and B-Cell Subsets That Expand in the Central and Peripheral Lymphoid Organs during the Establishment of Nut Allergy in an Adjuvant-Free Mouse Model
Figure 4
((a)–(c)) Analysis of central and peripheral phenotype of B lymphocytes from hazelnut allergic and nonallergic control mice. Groups of BALB/c ( to 7 per group) were sensitized with hazelnut protein and then confirmed for allergy by oral challenge. After 1 hour, bone marrow (a), spleen (b), and mesenteric lymph nodes (MLN) (c) were harvested and single-cell suspension prepared. Cells were stained with antibodies against surface markers CD80, CD62L, and CD73 and analyzed by flow cytometry. Differences between allergic and nonallergic mice data were compared by Student’s -test for significance ().
