Table of Contents
ISRN Virology
Volume 2013, Article ID 546503, 4 pages
Research Article

No Evidence of Human Papillomavirus in Patients with Breast Cancer in Hong Kong, Southern China

1Department of Surgery, The University of Hong Kong, Hong Kong
2The Hong Kong Hereditary and High Risk Breast Cancer Programme, The Hong Kong Hereditary Breast Cancer Family Registry, Hong Kong
3Breast Surgery Division, The University of Hong Kong, Hong Kong
4Department of Molecular Pathology, Hong Kong Sanatorium & Hospital, Hong Kong

Received 2 July 2013; Accepted 21 August 2013

Academic Editors: K. Ault and S.-L. You

Copyright © 2013 Ava Kwong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Several studies have suggested that viral oncogenesis is one of the etiologic factors of breast cancer, while others are provocative, however, their association remains controversial. The purpose of this study was to investigate whether the human papillomavirus (HPV) DNA is present in the blood and tissue samples of breast cancer patients in Hong Kong. A total of 102 patients with breast tumour tissues and adjacent normal tissues were available and recruited unselectively. Both DNA and RNA were extracted from those samples, and real-time quantitative PCR was performed to detect HPV-16, with 18 sequences targeting the E6 and L1 regions. Results showed that HPV DNA sequences were absent in all the blood and breast tissues. These data argue against the role of oncogenic HPV in the pathogenesis of breast cancer. Additional lines of evidence need to be obtained in order to assess the possibility of breast cancer prevention using HPV vaccines.