The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control
Table 1
Summary of phenotypes measured for different Not4 derivatives.
Phenotypes/Not4 derivatives
1–587
1–430
1–180
78–587
164A
L35A
Growth 30°C (doubling time in hrs in YPD)°
2.5
3.5
4.6
5
3.1
nd
Growth 37°C (YPD)°
Yes
Yes
No
No
Yes
Yes
Growth on CHX°
Yes
No
No
No
Yes
nd
Hydroxyurea or MMS&
Yes
nd
nd
nd
nd
No
Resistance to heat stress%
No
nd
nd
nd
Yes
Yes
SL or SS with ubp6 and/or doa4°
No
ss
sl
sl
ss
nd
Accumulation of polyubi°
No
No
Yes
Yes
Yes
nd
Unstable RP°
No
Yes
nd
Yes
No
nd
Salt-resistant RP-CP°
No
Yes
nd
Yes
No
nd
Ubiquitination of Rps7A*
Yes
Yes
Yes
No
No
nd
Toxic when overexpressed in hpr1 or nup116+
Yes
nd
nd
nd
nd
Yes
Binding to Ccr4-Not°
Yes
Weak
nd
Yes
Yes
nd
The phenotypes measured for different derivatives or Not4 in different studies: °[82], &[140], %[131], *[138], and +[142] are summarized here. Growth on different media or when combined with different mutants is indicated by yes or no and or by the doubling time in hours or finally by sl (synthetically lethal) or ss (synthetically sick). nd, not determined.
