Table of Contents
ISRN Allergy
Volume 2013, Article ID 561831, 6 pages
http://dx.doi.org/10.1155/2013/561831
Research Article

Sustained Elevation of Systemic Oxidative Stress and Inflammation in Exacerbation and Remission of Asthma

1Division of Respiratory Medicine, Departments of Medicine, The University of Hong Kong, Hong Kong
2Pharmacology & Pharmacy, The University of Hong Kong, Hong Kong
3Research Centre for Heart, Brain, Hormone and Healthy Aging, The University of Hong Kong, Hong Kong
4Room L8-40, Laboratory Block, LKS Faculty of Medicine Building, The University of Hong Kong, 21 Sassoon Road, Hong Kong
5Alice Ho Miu Ling Nethersole Hospital, Tai Po, Hong Kong

Received 4 June 2013; Accepted 1 August 2013

Academic Editors: S. Burastero, B. F. Gibbs, A. Lorentz, and T. Oga

Copyright © 2013 Judith C. W. Mak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Oxidative stress has been implicated in the pathogenesis of asthma. We aimed at investigating the biomarkers of oxidative stress, inflammation, and tissue damage in patients with asthma in acute exacerbation and remission. We recruited 18 asthmatics admitted to hospital with acute exacerbation and 18 healthy nonsmoking controls matched for age. We evaluated plasma levels of 8-isoprostane, C-reactive protein (CRP) and total matrix metalloproteinase- (MMP-) 9 by ELISA, and MMP-9 activity by zymographic analysis. Plasma levels of 8-isoprostane and CRP were significantly elevated in acute exacerbation and decreased in remission but remained significantly higher compared to healthy controls. The activities of pro-MMP-9 were also significantly higher in acute exacerbation and decreased in remission but remained significantly higher compared to healthy controls in parallel to plasma levels of total MMP-9. These data suggest that overproduction of MMP-9 along with highly elevated levels of oxidative stress and inflammation is implicated in asthma exacerbation and that measurements of these biomarkers can be a valid index in its management.