Table of Contents
ISRN Pharmacology
Volume 2013, Article ID 562905, 7 pages
Research Article

Amelioration of Collagen-Induced Arthritis in Female Dark Agouti Rats by Glucosamine Treatment

1School of Veterinary and Biomedical Sciences, James Cook University, Townsville, QLD 4811, Australia
2Department of Pathology, International Medical University, 57000 Kuala Lumpur, Malaysia

Received 7 December 2012; Accepted 8 January 2013

Academic Editors: M. Alkondon, G. Biala, G. Gervasini, and D. K. Miller

Copyright © 2013 Nagaraja Haleagrahara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study assessed the therapeutic efficacy of glucosamine hydrochloride against collagen-induced arthritis in female Dark Agouti rats (DA). Arthritis was induced by intradermaly injecting a collagen and complete Freund’s adjuvant suspension at multiple sites in the rat at a dose of 4 mg/kg of body weight and thereafter followed by two more boosters of the same dose, after the 1st week and 2nd week of primary immunization. After 21 days from the day of primary immunization, the arthritic group rats were given oral supplementation of glucosamine hydrochloride at a dose of 300 mg/kg of body weight until day 45. The arthritic group treated with glucosamine hydrochloride from day 21 to day 45 showed significant reduction in arthritic histopathological changes of the joints, reduction in paw thickness and also a significant decrease in C-reactive protein and TNF-alpha in the serum. Treatment with 300 mg/kg of glucosamine hydrochloride was able to reverse the arthritic changes, hence suggesting that glucosamine has a therapeutic effect against collagen-induced arthritis.