Table of Contents
ISRN Dentistry
Volume 2013, Article ID 573262, 10 pages
http://dx.doi.org/10.1155/2013/573262
Research Article

Synergistic Effects of Nonthermal Plasma and Disinfecting Agents against Dental Biofilms In Vitro

1Unit of Periodontology, Department of Restorative Dentistry, Periodontology and Endodontology, Ernst-Moritz-Arndt University Greifswald, Walther-Rathenau-Straße 49a, 17487 Greifswald, Germany
2Institute for Medical Informatics, Biometry and Epidemiology, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany
3Institute for Hygiene and Environmental Medicine, Ernst-Moritz-Arndt University Greifswald, Walther-Rathenau-Straße 49a, 17475 Greifswald, Germany
4Robert Koch Institut, Nordufer 20, 13353 Berlin, Germany
5Leibniz Institute for Plasma Science and Technology, Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany

Received 14 July 2013; Accepted 7 August 2013

Academic Editors: H. S. Cardash and G. H. Sperber

Copyright © 2013 Ina Koban et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. Dental biofilms play a major role in the pathogenesis of many dental diseases. In this study, we evaluated the synergistic effect of atmospheric pressure plasma and different agents in dentistry on the reduction of biofilms. Methods and Results. We used monospecies (S. mutans) and multispecies dental biofilm models grown on titanium discs in vitro. After treatment with one of the agents, the biofilms were treated with plasma. Efficacy of treatment was determined by the number of colony forming units (CFU) and by live-dead staining. For S. mutans biofilms no colonies could be detected after treatment with NaOCl or H2O2. For multispecies biofilms the combination with plasma achieved a higher CFU reduction than each agent alone. We found an additive antimicrobial effect between argon plasma and agents irrespective of the treatment order with cultivation technique. For EDTA and octenidine, antimicrobial efficacy assessed by live-dead staining differed significantly between the two treatment orders ( ). Conclusions. The effective treatment of dental biofilms on titanium discs with atmospheric pressure plasma could be increased by adding agents in vitro.