Age-Related Differences in Response to High-Fat Feeding on Adipose Tissue and Metabolic Profile in ZDSD Rats

Table 2

Transcript abundance of Wnt signaling intermediates in AT depots of ZDSD rats fed HFD beginning at 63, 84, or 112 d after parturition.

	Subcutaneous				Epididymal
	HFD 63d	HFD 84 d	HFD 112 d	HFD 63 d	HFD 84 d	HFD 112 d
Adipogenic transcription factors
PPAR𝛾	1.18	1.56	1.23	1.17	1.20	1.43
C/EBP𝛼	1.14	1.18	1.15	0.93	1.20	1.26
Wnt ligands
Wnt1	0.33^a	0.82^b	0.88^b	0.88^a	1.56^a	5.36^b
Wnt3a	0.92^a	1.95^b	3.02^c	1.12^a	1.32^a	3.92^b
Wnt10b	0.62^a	0.44^a	2.97^b	0.78^a	0.47^a	3.94^b
Extracellular Wnt receptors
Fzd1	0.93^a	1.17^ab	1.93^b	1.29	1.45	1.44
Fzd2	0.53^a	1.00^ab	1.82^b	2.69^a	2.51^a	10.74^b
Fzd5	1.06^a	1.46^ab	1.95^b	0.62^a	1.08^ab	1.38^b
Intracellular Wnt signaling intermediates
Gsk3𝛽	1.02	1.43	0.89	4.39^a	4.17^a	8.20^b
𝛽-catenin	1.09	1.32	1.34	2.44^a	2.41^a	6.44^b
TCF/LEF	0.62	0.82	0.78	0.85	1.28	1.27

∗Data are expressed as fold change relative to animals fed LFD. Letters represent significant difference among treatment groups as determined by comparison of normalized Ct values (target Ct-housekeeping Ct) with paired 𝑡-test.